Overexpression of the potassium channel TPKb in small vacuoles confers osmotic and drought tolerance to rice
© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 209(2016), 3 vom: 01. Feb., Seite 1040-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2016
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article TPKb drought ion channel osmotic stress potassium rice (Oryza sativa) small vacuole Plant Proteins Potassium Channels mehr... |
| Zusammenfassung: | © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Potassium (K(+) ) is the most important cationic nutrient for all living organisms. Vacuolar two-pore K(+) (TPK) channels are important players in the regulation of cellular levels of K(+) but have not been characterised in rice. In order to assess the role of OsTPKb, a K(+) selective ion channel predominantly expressed in the tonoplast of small vacuoles, we generated overexpressing (OX) lines using a constitutive promoter and compared their phenotypes with control plants. Relative to control plants, OX lines showed better growth when exposed to low-K(+) or water stress conditions. K(+) uptake was greater in OX lines which may be driven by increased AKT1 and HAK1 activity. The enhanced K(+) uptake led to tissue K(+) levels that were raised in roots and shoots. Furthermore, energy dispersive X-ray (EDX) analyses showed a higher cytoplasm: vacuole K(+) ratio which is likely to contribute to the increased stress tolerance. In all, the data suggest that TPKb can alter the K(+) status of small vacuoles, which is important for general cellular K(+) homeostasis which, in turn, affects stress tolerance |
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| Beschreibung: | Date Completed 13.12.2016 Date Revised 30.09.2020 published: Print-Electronic GENBANK: AK109604 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.13708 |