The tomato plastidic fructokinase SlFRK3 plays a role in xylem development

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 209(2016), 4 vom: 14. März, Seite 1484-95
1. Verfasser:	Stein, Ofer (VerfasserIn)
Weitere Verfasser:	Damari-Weissler, Hila, Secchi, Francesca, Rachmilevitch, Shimon, German, Marcelo A, Yeselson, Yelena, Amir, Rachel, Schaffer, Arthur, Holbrook, N Michele, Aloni, Roni, Zwieniecki, Maciej A, Granot, David
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't RNAi fructokinase fructose plastid sucrose tomato (Solanum lycopersicon) xylem fiber xylem vessel mehr... Plant Proteins Water 059QF0KO0R Fructokinases EC 2.7.1.- EC 2.7.1.4

Beschreibung
Zusammenfassung:	© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Plants have two kinds of fructokinases (FRKs) that catalyze the key step of fructose phosphorylation, cytosolic and plastidic. The major cytosolic tomato FRK, SlFRK2, is essential for the development of xylem vessels. In order to study the role of SlFRK3, which encodes the only plastidic FRK, we generated transgenic tomato (Solanum lycopersicon) plants with RNAi suppression of SlFRK3 as well as plants expressing beta-glucoronidase (GUS) under the SlFRK3 promoter. GUS staining indicated SlFRK3 expression in vascular tissues of the leaves and stems, including cambium, differentiating xylem, young xylem fibers and phloem companion cells. Suppression of SlFRK3 reduced the stem xylem area, stem and root water conductance, and whole-plant transpiration, with minor effects on plant development. However, suppression of SlFRK3 accompanied by partial suppression of SlFRK2 induced significant growth-inhibition effects, including the wilting of mature leaves. Grafting experiments revealed that these growth effects are imposed primarily by the leaves, whose petioles had unlignified, thin-walled xylem fibers with collapsed parenchyma cells around the vessels. A cross between the SlFRK2-antisense and SlFRK3-RNAi lines exhibited similar wilting and anatomical effects, confirming that these effects are the result of the combined suppression of SlFRK3 and SlFRK2. These results demonstrate a role of the plastidic SlFRK3 in xylem development and hydraulic conductance
Beschreibung:	Date Completed 19.12.2016 Date Revised 07.12.2022 published: Print-Electronic ErratumIn: New Phytol. 2016 Jul;211(1):371. - PMID 27240711 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.13705