MADS1, a novel MADS-box protein, is involved in the response of Nicotiana benthamiana to bacterial harpin(Xoo)

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 67(2016), 1 vom: 13. Jan., Seite 131-41
1. Verfasser:	Zhang, Huajian (VerfasserIn)
Weitere Verfasser:	Teng, Wenjun, Liang, Jingang, Liu, Xinyu, Zhang, Haifeng, Zhang, Zhengguang, Zheng, Xiaobo
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Elicitor MADS-box Nicotiana benthamiana harpin hypersensitive response stomatal closure. Bacterial Outer Membrane Proteins DNA, Complementary mehr... MADS Domain Proteins Plant Proteins

Beschreibung
Zusammenfassung:	© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. MADS-box transcription factor genes are well known for their role in floral organ and seed development. In this study, a novel MADS-box-containing gene, designated NbMADS1, was isolated from leaves of Nicotiana benthamiana. The full-length cDNA was 666 bp and encoded a putative polypeptide of 221 aa with a mass of 24.3 kDa. To assess the role of NbMADS1 in the defence response to bacterial harpin(Xoo), an elicitor of the hypersensitive response, a loss-of-function experiment was performed in N. benthamiana plants using virus-induced gene silencing. Analyses of electrolyte leakage revealed more extensive cell death in the control plants than in NbMADS1-silenced plants. The NbMADS1-silenced plants showed impaired harpin(Xoo)-induced stomatal closure, decreased harpin(Xoo)-induced production of hydrogen peroxide (H2O2) and nitric oxide (NO) in guard cells, and reduced harpin(Xoo)-induced resistance to Phytophthora nicotianae. The compromised stomatal closure observed in the NbMADS1-silenced plants was inhibited by the application of H2O2 and sodium nitroprusside (an NO donor). Taken together, these results demonstrate that the NbMADS1-H2O2-NO pathway mediates multiple harpin(Xoo)-triggered responses, including stomatal closure, hypersensitive cell death, and defence-related gene expression, suggesting that NbMADS1 plays an important role in regulating the response to harpin(Xoo) in N. benthamiana plants
Beschreibung:	Date Completed 23.09.2016 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erv448