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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2015.09.004
|2 doi
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|a pubmed24n0844.xml
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|a (PII)S0981-9428(15)30106-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Walker, Robert P
|e verfasserin
|4 aut
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|a Phosphoenolpyruvate carboxykinase and gluconeogenesis in grape pericarp
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.09.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Masson SAS. All rights reserved.
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|a Glycolysis from sugars is necessary at all stages of development of grape pericarp, and this raises the question as to why gluconeogenesis from malate occurs. Phosphoenolpyruvate carboxykinase (PEPCK) is required for gluconeogenesis in grape pericarp. In this study we determined the abundance of PEPCK protein and activity in different parts of grape pericarp during its development. Both PEPCK protein and activity were present throughout development, however, in both the skin and the flesh their abundance increased greatly at the start of ripening. This coincided with the onset of the decrease in the malate content of the berry. The location of PEPCK in the pericarp at different stages of development was determined using both immunohistochemistry and dissection. We provide a possible explanation for the occurrence of gluconeogenesis in grape pericarp
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gluconeogenesis
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|a Grape
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|a Malate
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|a Organic acids
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|a Phosphoenolpyruvate carboxykinase
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|a Vitis vinifera L.
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|a Malates
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Phosphoenolpyruvate
|2 NLM
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|a 73-89-2
|2 NLM
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|a malic acid
|2 NLM
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|a 817L1N4CKP
|2 NLM
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|a Phosphoenolpyruvate Carboxylase
|2 NLM
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|a EC 4.1.1.31
|2 NLM
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|a Battistelli, Alberto
|e verfasserin
|4 aut
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|a Moscatello, Stefano
|e verfasserin
|4 aut
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|a Técsi, László
|e verfasserin
|4 aut
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|a Leegood, Richard C
|e verfasserin
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|a Famiani, Franco
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 97(2015) vom: 21. Dez., Seite 62-9
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:97
|g year:2015
|g day:21
|g month:12
|g pages:62-9
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|u http://dx.doi.org/10.1016/j.plaphy.2015.09.004
|3 Volltext
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