Mitochondrial alternative oxidase is involved in both compatible and incompatible host-virus combinations in Nicotiana benthamiana

Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Détails bibliographiques
Publié dans:Plant science : an international journal of experimental plant biology. - 1985. - 239(2015) vom: 20. Okt., Seite 26-35
Auteur principal: Zhu, Feng (Auteur)
Autres auteurs: Deng, Xing-Guang, Xu, Fei, Jian, Wei, Peng, Xing-Ji, Zhu, Tong, Xi, De-Hui, Lin, Hong-Hui
Format: Article en ligne
Langue:English
Publié: 2015
Accès à la collection:Plant science : an international journal of experimental plant biology
Sujets:Journal Article Research Support, Non-U.S. Gov't Alternative oxidase (AOX) Systemic resistance Tobacco mosaic virus (TMV) Turnip crinkle virus (TCV) Virus-induced gene-silencing (VIGS) Mitochondrial Proteins Plant Proteins Oxidoreductases plus... EC 1.- alternative oxidase
Description
Résumé:Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
The alternative oxidase (AOX) functions in the resistance to biotic stress. However, the mechanisms of AOX in the systemic antiviral defense response and N (a typical resistance gene)-mediated resistance to Tobacco mosaic virus (TMV) are elusive. A chemical approach was undertaken to investigate the role of NbAOX in the systemic resistance to RNA viruses. Furthermore, we used a virus-induced gene-silencing (VIGS)-based genetics approach to investigate the function of AOX in the N-mediated resistance to TMV. The inoculation of virus significantly increased the NbAOX transcript and protein levels and the cyanide-resistant respiration in the upper un-inoculated leaves. Pretreatment with potassium cyanide greatly increased the plant's systemic resistance, whereas the application of salicylhydroxamic acid significantly compromised the plant's systemic resistance. Additionally, in NbAOX1a-silenced N-transgenic Nicotiana benthamiana plants, the inoculated leaf collapsed and the movement of TMV into the systemic tissue eventually led to the spreading of HR-PCD and the death of the whole plant. The hypersensitive response marker gene HIN1 was significantly increased in the NbAOX1a-silenced plants. Significant amounts of TMV-CP mRNA and protein were detected in the NbAOX1a-silenced plants but not in the control plants. Overall, evidence is provided that AOX plays important roles in both compatible and incompatible plant-virus combinations
Description:Date Completed 12.07.2016
Date Revised 13.12.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2015.07.009