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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2015.08.004
|2 doi
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|a pubmed24n0842.xml
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|a (DE-627)NLM252693213
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|a (NLM)26363719
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|a (PII)S0176-1617(15)00204-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chen, Hsien-Jung
|e verfasserin
|4 aut
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| 245 |
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|a Sweet potato cysteine proteases SPAE and SPCP2 participate in sporamin degradation during storage root sprouting
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.04.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier GmbH. All rights reserved.
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|a Sweet potato sporamins are trypsin inhibitors and exhibit strong resistance to digestion by pepsin, trypsin and chymotrypsin. In addition, they constitute the major storage proteins in the sweet potato and, after degradation, provide nitrogen as a nutrient for seedling regrowth in sprouting storage roots. In this report, four cysteine proteases-one asparaginyl endopeptidase (SPAE), two papain-like cysteine proteases (SPCP1 and SPCP2), and one granulin-containing cysteine protease (SPCP3)-were studied to determine their association with sporamin degradation in sprouting storage roots. Sporamin degradation became significant in the flesh of storage roots starting from week 4 after sprouting and this correlated with expression levels of SPAE and SPCP2, but not of SPCP1 and SPCP3. In the outer flesh near the skin, sporamin degradation was more evident and occurred earlier than in the inner flesh of storage roots. Degradation of sporamins in the outer flesh was inversely correlated with the distance of the storage root from the sprout. Exogenous application of SPAE and SPCP2, but not SPCP3, fusion proteins to crude extracts of the outer flesh (i.e., extracted from a depth of 0.3cm and within 2cm of one-week-old sprouts) promoted in vitro sporamin degradation in a dose-dependent manner. Pre-treatment of SPAE and SPCP2 fusion proteins at 95°C for 5min prior to their application to the crude extracts reduced sporamin degradation. These data show that sweet potato asparaginyl endopeptidase SPAE and papain-like cysteine protease SPCP2 participate in sporamin degradation during storage root sprouting
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cysteine protease
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|a Sporamin
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|a Sprout
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|a Sweet potato
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|a Trypsin inhibitor
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|a Plant Proteins
|2 NLM
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|a Trypsin Inhibitors
|2 NLM
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|a sporamin protein, Ipomoea batatas
|2 NLM
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| 650 |
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|a Cysteine Proteases
|2 NLM
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|a EC 3.4.-
|2 NLM
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|a Cysteine Endopeptidases
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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| 650 |
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|a asparaginylendopeptidase
|2 NLM
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| 650 |
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|a EC 3.4.22.34
|2 NLM
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| 700 |
1 |
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|a Liang, Shu-Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Guan-Jhong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Yaw-Huei
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 186-187(2015) vom: 15. Aug., Seite 39-49
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:186-187
|g year:2015
|g day:15
|g month:08
|g pages:39-49
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2015.08.004
|3 Volltext
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|a AR
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|d 186-187
|j 2015
|b 15
|c 08
|h 39-49
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