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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2015.09.002
|2 doi
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|a pubmed24n0842.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Ramesh, Manish
|e verfasserin
|4 aut
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| 245 |
1 |
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|a High-throughput sequencing reveals an altered T cell repertoire in X-linked agammaglobulinemia
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.02.2016
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|a Date Revised 16.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Inc. All rights reserved.
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|a To examine the T cell receptor structure in the absence of B cells, the TCR β CDR3 was sequenced from DNA of 15 X-linked agammaglobulinemia (XLA) subjects and 18 male controls, using the Illumina HiSeq platform and the ImmunoSEQ analyzer. V gene usage and the V-J combinations, derived from both productive and non-productive sequences, were significantly different between XLA samples and controls. Although the CDR3 length was similar for XLA and control samples, the CDR3 region of the XLA T cell receptor contained significantly fewer deletions and insertions in V, D, and J gene segments, differences intrinsic to the V(D)J recombination process and not due to peripheral T cell selection. XLA CDR3s demonstrated fewer charged amino acid residues, more sharing of CDR3 sequences, and almost completely lacked a population of highly modified Vβ gene segments found in control DNA, suggesting both a skewed and contracted T cell repertoire in XLA
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Amino acid sequence
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|a High throughput sequencing
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|a Junctional diversity
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|a T cell receptor
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| 650 |
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|a XLA
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| 650 |
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|a Immunoglobulin Variable Region
|2 NLM
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| 650 |
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|a Receptor-CD3 Complex, Antigen, T-Cell
|2 NLM
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| 650 |
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|a Receptors, Antigen, T-Cell
|2 NLM
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Simchoni, Noa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hamm, David
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cunningham-Rundles, Charlotte
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 161(2015), 2 vom: 15. Dez., Seite 190-6
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:161
|g year:2015
|g number:2
|g day:15
|g month:12
|g pages:190-6
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2015.09.002
|3 Volltext
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