Identification, evolution, and expression partitioning of miRNAs in allopolyploid Brassica napus

Identification, evolution, and expression partitioning of miRNAs in allopolyploid Brassica napus

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 66(2015), 22 vom: 03. Dez., Seite 7241-53
1. Verfasser: Shen, Enhui (VerfasserIn)
Weitere Verfasser: Zou, Jun, Hubertus Behrens, Falk, Chen, Li, Ye, Chuyu, Dai, Shutao, Li, Ruiyan, Ni, Meng, Jiang, Xiaoxue, Qiu, Jie, Liu, Yang, Wang, Weidi, Zhu, Qian-Hao, Chalhoub, Boulos, Bancroft, Ian, Meng, Jinling, Cai, Daguang, Fan, Longjiang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Brassica napus allopolyploid evolution expression partitioning microRNA. MicroRNAs RNA, Plant
Beschreibung
Zusammenfassung:© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
The recently published genome of Brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of miRNAs in oilseed rape. In this study, 12 small RNA libraries from two B. napus cultivars (Tapidor and Ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced B. napus genome, together with genomes of its progenitors Brassica rapa and Brassica oleracea. A total of 645 miRNAs including 280 conserved and 365 novel miRNAs were identified. Comparative analysis revealed a high level of genomic conservation of MIRNAs (75.9%) between the subgenomes of B. napus and its two progenitors' genomes, and MIRNA lost/gain events (133) occurred in B. napus after its speciation. Furthermore, significant partitioning of miRNA expressions between the two subgenomes in B. napus was detected. The data of degradome sequencing, miRNA-mediated cleavage, and expression analyses support specific interactions between miRNAs and their targets in the modulation of diverse physiological processes in roots and leaves, as well as in biosynthesis of, for example, glucosinolates and lipids in oilseed rape. These data provide a first genome-wide view on the origin, evolution, and genomic organization of B. napus MIRNAs
Beschreibung:Date Completed 12.10.2016
Date Revised 29.01.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/erv420