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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erv416
|2 doi
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|a pubmed24n0842.xml
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|a (DE-627)NLM252609891
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|a (NLM)26355148
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Tuo
|e verfasserin
|4 aut
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| 245 |
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|a Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 06.09.2016
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|a Date Revised 11.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Until recently, the 3D architecture of plant cell walls was poorly understood due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics, and intermolecular interactions of the wall polysaccharides in these insoluble biomolecular mixtures. We introduced multidimensional solid-state NMR (SSNMR) spectroscopy, coupled with (13)C labelling of whole plants, to determine the spatial arrangements of macromolecules in near-native plant cell walls. Here we review key evidence from 2D and 3D correlation NMR spectra that show relatively few cellulose-hemicellulose cross peaks but many cellulose-pectin cross peaks, indicating that cellulose microfibrils are not extensively coated by hemicellulose and all three major polysaccharides exist in a single network rather than two separate networks as previously proposed. The number of glucan chains in the primary-wall cellulose microfibrils has been under active debate recently. We show detailed analysis of quantitative (13)C SSNMR spectra of cellulose in various wild-type (WT) and mutant Arabidopsis and Brachypodium primary cell walls, which consistently indicate that primary-wall cellulose microfibrils contain at least 24 glucan chains
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Review
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|a Arabidopsis
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|a Brachypodium
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|a cellulose microfibrils
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|a dynamics
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|a intermolecular contact
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|a multidimensional correlation NMR.
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|a Plant Proteins
|2 NLM
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|a Polysaccharides
|2 NLM
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|a hemicellulose
|2 NLM
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|a 8024-50-8
|2 NLM
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| 650 |
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|a Pectins
|2 NLM
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| 650 |
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|a 89NA02M4RX
|2 NLM
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|a Cellulose
|2 NLM
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| 650 |
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|a 9004-34-6
|2 NLM
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| 700 |
1 |
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|a Hong, Mei
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 2 vom: 15. Jan., Seite 503-14
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:67
|g year:2016
|g number:2
|g day:15
|g month:01
|g pages:503-14
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|u http://dx.doi.org/10.1093/jxb/erv416
|3 Volltext
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