A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in...

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Veröffentlicht in:BioTechniques. - 1993. - 59(2015), 3 vom: 08. Sept., Seite 153-5
1. Verfasser: Nakagawa, Akifumi (VerfasserIn)
Weitere Verfasser: Alt, Kate Von, Lillemoe, Keith D, Castillo, Carlos Fernández-Del, Warshaw, Andrew L, Liss, Andrew S
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't FFPE GFP natural fluorescence Fluorescent Dyes Luminescent Proteins Recombinant Proteins Green Fluorescent Proteins 147336-22-9