Application of an RNA amplification method for reliable single-cell transcriptome analysis

Diverse cell types have unique transcriptional signatures that are best interrogated at single-cell resolution. Here we describe a novel RNA amplification approach that allows for high fidelity gene profiling of individual cells. This technique significantly diminishes the problem of 3' bias, e...

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Publié dans:	BioTechniques. - 1991. - 59(2015), 3 vom: 08. Sept., Seite 137-48
Auteur principal:	Suslov, Oleg (Auteur)
Autres auteurs:	Silver, Daniel J, Siebzehnrubl, Florian A, Orjalo, Arturo, Ptitsyn, Andrey, Steindler, Dennis A
Format:	Article en ligne
Langue:	English
Publié:	2015
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't RNA amplification SVZ single-cell analysis stem and progenitor cells AC133 Antigen Antigens, CD DNA-Binding Proteins plus... Eye Proteins Glial Fibrillary Acidic Protein Glycoproteins Homeodomain Proteins Idb1 protein, mouse Inhibitor of Differentiation Protein 1 Membrane Proteins Nerve Tissue Proteins NeuN protein, mouse Nuclear Proteins Numb protein, mouse PAX6 Transcription Factor PAX6 protein, human Paired Box Transcription Factors Pax6 protein, mouse Peptides Repressor Proteins glial fibrillary astrocytic protein, mouse Green Fluorescent Proteins 147336-22-9 RNA 63231-63-0 EGFR protein, mouse EC 2.7.10.1 ErbB Receptors