Application of an RNA amplification method for reliable single-cell transcriptome analysis
Diverse cell types have unique transcriptional signatures that are best interrogated at single-cell resolution. Here we describe a novel RNA amplification approach that allows for high fidelity gene profiling of individual cells. This technique significantly diminishes the problem of 3' bias, e...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 59(2015), 3 vom: 08. Sept., Seite 137-48
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| 1. Verfasser: |
Suslov, Oleg
(VerfasserIn) |
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| Weitere Verfasser: |
Silver, Daniel J,
Siebzehnrubl, Florian A,
Orjalo, Arturo,
Ptitsyn, Andrey,
Steindler, Dennis A |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
RNA amplification
SVZ
single-cell analysis
stem and progenitor cells
AC133 Antigen
Antigens, CD
DNA-Binding Proteins
mehr...
Eye Proteins
Glial Fibrillary Acidic Protein
Glycoproteins
Homeodomain Proteins
Idb1 protein, mouse
Inhibitor of Differentiation Protein 1
Membrane Proteins
Nerve Tissue Proteins
NeuN protein, mouse
Nuclear Proteins
Numb protein, mouse
PAX6 Transcription Factor
PAX6 protein, human
Paired Box Transcription Factors
Pax6 protein, mouse
Peptides
Repressor Proteins
glial fibrillary astrocytic protein, mouse
Green Fluorescent Proteins
147336-22-9
RNA
63231-63-0
EGFR protein, mouse
EC 2.7.10.1
ErbB Receptors |
|---|