Phosphorothioate-based DNA recombination : an enzyme-free method for the combinatorial assembly of multiple DNA fragments
Rational guided generation of protein chimeras has developed into an attractive approach in protein engineering for tailoring catalytic and biophysical properties of enzymes. Combinatorial recombination of structural elements or whole protein domains is still technically challenging due to sequence...
| Veröffentlicht in: | BioTechniques. - 1991. - 52(2012), 5 vom: 01. Mai |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't PTRec directed evolution phosphorothioate protein engineering recombination Phosphates phosphorodithioic acid 8056RR93HU mehr... |
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