Improved split-ubiquitin screening technique to identify surface membrane protein-protein interactions
Yeast-based methods are still the workhorse for the detection of protein-protein interactions (PPIs) in vivo. Yeast two-hybrid (Y2H) systems, however, are limited to screening for a specific group of molecules that interact in a particular cell compartment. For this reason, the split-ubiquitin syste...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 59(2015), 2 vom: 21. Aug., Seite 63-73
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| 1. Verfasser: |
Ivanusic, Daniel
(VerfasserIn) |
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| Weitere Verfasser: |
Heinisch, Jürgen J,
Eschricht, Magdalena,
Laube, Ulrike,
Denner, Joachim |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
protein-protein interactions
receptors
split-ubiquitin system
Membrane Proteins
SLG1 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Ubiquitin |
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