A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 209(2016), 1 vom: 06. Jan., Seite 161-76
1. Verfasser: Bonnot, Clémence (VerfasserIn)
Weitere Verfasser: Pinson, Benoît, Clément, Mathilde, Bernillon, Stéphane, Chiarenza, Serge, Kanno, Satomi, Kobayashi, Natsuko, Delannoy, Etienne, Nakanishi, Tomoko M, Nussaume, Laurent, Desnos, Thierry
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Oryza sativa PHOSTIN chemical genetics phosphate homeostasis phosphate starvation phr1;phl1 mutant Arabidopsis Proteins mehr... Isoxazoles PHO1 protein, Arabidopsis PHOSTIN compound Phosphates Small Molecule Libraries Transcription Factors UBC24 protein, Arabidopsis EC 2.3.2.23 Ubiquitin-Conjugating Enzymes
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520 |a Plants display numerous strategies to cope with phosphate (Pi)-deficiency. Despite multiple genetic studies, the molecular mechanisms of low-Pi-signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi-starvation in Arabidopsis. We assessed the effects of PSN and structural analogues on the induction of Pi-deficiency responses in mutants and wild-type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass-spectrophotometry. We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi-starvation effects. Nevertheless, albeit that the transcriptional activation of low-Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects. The range of local and systemic responses to Pi-starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi-starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis thaliana 
650 4 |a Oryza sativa 
650 4 |a PHOSTIN 
650 4 |a chemical genetics 
650 4 |a phosphate homeostasis 
650 4 |a phosphate starvation 
650 4 |a phr1;phl1 mutant 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Isoxazoles  |2 NLM 
650 7 |a PHO1 protein, Arabidopsis  |2 NLM 
650 7 |a PHOSTIN compound  |2 NLM 
650 7 |a Phosphates  |2 NLM 
650 7 |a Small Molecule Libraries  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a UBC24 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.3.2.23  |2 NLM 
650 7 |a Ubiquitin-Conjugating Enzymes  |2 NLM 
650 7 |a EC 2.3.2.23  |2 NLM 
700 1 |a Pinson, Benoît  |e verfasserin  |4 aut 
700 1 |a Clément, Mathilde  |e verfasserin  |4 aut 
700 1 |a Bernillon, Stéphane  |e verfasserin  |4 aut 
700 1 |a Chiarenza, Serge  |e verfasserin  |4 aut 
700 1 |a Kanno, Satomi  |e verfasserin  |4 aut 
700 1 |a Kobayashi, Natsuko  |e verfasserin  |4 aut 
700 1 |a Delannoy, Etienne  |e verfasserin  |4 aut 
700 1 |a Nakanishi, Tomoko M  |e verfasserin  |4 aut 
700 1 |a Nussaume, Laurent  |e verfasserin  |4 aut 
700 1 |a Desnos, Thierry  |e verfasserin  |4 aut 
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