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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1080/08997659.2015.1047538
|2 doi
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|a pubmed25n0837.xml
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|a (NLM)26223267
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|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Payattikul, Penpan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development of a PCR Assay Based on a Single-Base Pair Substitution for the Detection of Aeromonas caviae by Targeting the gyrB Gene
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 30.11.2015
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. To facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrB gene for use in a PCR assay. The technique was able to detect 100% (29/29) of the A. caviae strains tested using either of two sets of primers (designated ACF1-ACR and ACF3-ACR), which produced 293-bp and 206-bp amplicons, respectively. Another set of primers (designated ACF2-ACR) yielded a 237-bp amplicon and exhibited 90% (26/29) positive results with respect to A. caviae. None of the primer sets exhibited cross-reactivity with 12 non-A. caviae isolates and 52 other non-Aeromonas bacteria. The detection limit using the ACF2-ACR and ACF3-ACR primer sets in pure culture was 1.6 × 10(3) CFU/mL, or 6 CFU per reaction, whereas that of the ACF1-ACR primer set was 1.6 × 10(4) CFU/mL, or 60 CFU per reaction. In the case of spiked Nile Tilapia Oreochromis niloticus, the sensitivity of all primer sets without enrichment was 1.8 × 10(4) CFU/g, or 30 CFU per reaction. Primer set ACF3-ACR was the best for a PCR assay targeting the gyrB gene, and the PCR technique developed was rapid, specific, and sensitive for the identification of A. caviae
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bacterial Proteins
|2 NLM
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|a DNA Gyrase
|2 NLM
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| 650 |
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7 |
|a EC 5.99.1.3
|2 NLM
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| 700 |
1 |
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|a Longyant, Siwaporn
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sithigorngul, Paisarn
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chaivisuthangkura, Parin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 27(2015), 3 vom: 30. Sept., Seite 164-71
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnas
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| 773 |
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|g volume:27
|g year:2015
|g number:3
|g day:30
|g month:09
|g pages:164-71
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|u http://dx.doi.org/10.1080/08997659.2015.1047538
|3 Volltext
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