|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM251320219 |
| 003 |
DE-627 |
| 005 |
20231224161738.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erv370
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0837.xml
|
| 035 |
|
|
|a (DE-627)NLM251320219
|
| 035 |
|
|
|a (NLM)26220082
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Yadav, Dinesh
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 12.08.2016
|
| 500 |
|
|
|a Date Revised 17.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Heterotrimeric nuclear factors Y (NF-Ys) are involved in regulation of various vital functions in all eukaryotic organisms. Although a number of NF-Y subunits have been characterized in model plants, only a few have been functionally evaluated in crops. In this work, a number of genes encoding NF-YB and NF-YC subunits were isolated from drought-tolerant wheat (Triticum aestivum L. cv. RAC875), and the impact of the overexpression of TaNF-YB4 in the Australian wheat cultivar Gladius was investigated. TaNF-YB4 was isolated as a result of two consecutive yeast two-hybrid (Y2H) screens, where ZmNF-YB2a was used as a starting bait. A new NF-YC subunit, designated TaNF-YC15, was isolated in the first Y2H screen and used as bait in a second screen, which identified two wheat NF-YB subunits, TaNF-YB2 and TaNF-YB4. Three-dimensional modelling of a TaNF-YB2/TaNF-YC15 dimer revealed structural determinants that may underlie interaction selectivity. The TaNF-YB4 gene was placed under the control of the strong constitutive polyubiquitin promoter from maize and introduced into wheat by biolistic bombardment. The growth and yield components of several independent transgenic lines with up-regulated levels of TaNF-YB4 were evaluated under well-watered conditions (T1-T3 generations) and under mild drought (T2 generation). Analysis of T2 plants was performed in large deep containers in conditions close to field trials. Under optimal watering conditions, transgenic wheat plants produced significantly more spikes but other yield components did not change. This resulted in a 20-30% increased grain yield compared with untransformed control plants. Under water-limited conditions transgenic lines maintained parity in yield performance
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Drought
|
| 650 |
|
4 |
|a NF-Y genes
|
| 650 |
|
4 |
|a grain yield
|
| 650 |
|
4 |
|a plant development
|
| 650 |
|
4 |
|a protein–protein interactions
|
| 650 |
|
4 |
|a wheat
|
| 650 |
|
7 |
|a CCAAT-Binding Factor
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Transcription Factors
|2 NLM
|
| 650 |
|
7 |
|a nuclear factor Y
|2 NLM
|
| 650 |
|
7 |
|a Polyubiquitin
|2 NLM
|
| 650 |
|
7 |
|a 120904-94-1
|2 NLM
|
| 700 |
1 |
|
|a Shavrukov, Yuri
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bazanova, Natalia
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chirkova, Larissa
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Borisjuk, Nikolai
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kovalchuk, Nataliya
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ismagul, Ainur
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Parent, Boris
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Langridge, Peter
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hrmova, Maria
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lopato, Sergiy
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 66(2015), 21 vom: 29. Nov., Seite 6635-50
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:66
|g year:2015
|g number:21
|g day:29
|g month:11
|g pages:6635-50
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erv370
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 66
|j 2015
|e 21
|b 29
|c 11
|h 6635-50
|