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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1080/08997659.2015.1037468
|2 doi
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|a eng
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| 100 |
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|a Plaon, Saranya
|e verfasserin
|4 aut
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|a Rapid and Sensitive Detection of Vibrio alginolyticus by Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick Targeted to the rpoX Gene
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|c 2015
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|a Text
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|a Date Completed 30.11.2015
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Vibrio alginolyticus is a major bacterial pathogen causing disease in marine animals. The present study aimed to develop a loop-mediated isothermal amplification (LAMP) coupled with a lateral flow dipstick (LFD) for rapid and simple visual detection of V. alginolyticus-specific amplicons. The biotin-labeled LAMP amplicons from the targeted portion of a gene encoding rpoS-like sigma factor (rpoX) were generated at 60°C for 1 h and then hybridized with a fluorescein isothiocyanate-labeled probe for 5 min for visual detection with LFD. In pure cultures, the detection limit of the LAMP-LFD technique for V. alginolyticus was 1.8 × 10(2) CFU/mL while that of PCR was 1.8 × 10(3) CFU/mL. In spiked whiteleg shrimp samples Penaeus vannamei, the sensitivity for V. alginolyticus detection was 2 × 10(3) CFU/g (equivalent to 4 CFU per reaction) while PCR was 10 times less sensitive. The LAMP-LFD method for V. alginolyticus correctly identified 21 isolates of V. alginolyticus but did not recognize 23 non-V. alginolyticus Vibrio isolates and 15 non-Vibrio isolates. In summary, this LAMP-LFD method targeted to the rpoX gene is a convenient assay for specific identification of V. alginolyticus with high sensitivity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bacterial Proteins
|2 NLM
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|a Molecular Probes
|2 NLM
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|a Longyant, Siwaporn
|e verfasserin
|4 aut
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|a Sithigorngul, Paisarn
|e verfasserin
|4 aut
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|a Chaivisuthangkura, Parin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 27(2015), 3 vom: 28. Sept., Seite 156-63
|w (DE-627)NLM098202006
|x 1548-8667
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| 773 |
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|g volume:27
|g year:2015
|g number:3
|g day:28
|g month:09
|g pages:156-63
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|u http://dx.doi.org/10.1080/08997659.2015.1037468
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