|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM250980959 |
| 003 |
DE-627 |
| 005 |
20250104232240.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erv348
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1651.xml
|
| 035 |
|
|
|a (DE-627)NLM250980959
|
| 035 |
|
|
|a (NLM)26184996
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Nguyen, Thu-Phuong
|e verfasserin
|4 aut
|
| 245 |
1 |
2 |
|a A role for seed storage proteins in Arabidopsis seed longevity
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 19.07.2016
|
| 500 |
|
|
|a Date Revised 04.01.2025
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana genotypes, that carry introgression fragments at the position of seed longevity quantitative trait loci and as a result display different levels of seed longevity, was investigated. Seeds at two physiological states, after-ripened seeds that had the full germination ability and aged (stored) seeds of which the germination ability was severely reduced, were compared. Aged dry seed proteomes were markedly different from the after-ripened and reflected the seed longevity level of the four genotypes, despite the fact that dry seeds are metabolically quiescent. Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity. Moreover, a new role for seed storage proteins (SSPs) was identified in dry seeds during ageing. Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds. These results confirm that oxidation is involved in seed deterioration and that SSPs buffer the seed from oxidative stress, thus protecting important proteins required for seed germination and seedling formation
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Arabidopsis
|
| 650 |
|
4 |
|a carbonylation
|
| 650 |
|
4 |
|a proteomics
|
| 650 |
|
4 |
|a reactive oxygen species
|
| 650 |
|
4 |
|a seed longevity
|
| 650 |
|
4 |
|a seed storage proteins.
|
| 650 |
|
7 |
|a Proteome
|2 NLM
|
| 650 |
|
7 |
|a Seed Storage Proteins
|2 NLM
|
| 700 |
1 |
|
|a Cueff, Gwendal
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hegedus, Dwayne D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Rajjou, Loïc
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bentsink, Leónie
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 66(2015), 20 vom: 01. Okt., Seite 6399-413
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:66
|g year:2015
|g number:20
|g day:01
|g month:10
|g pages:6399-413
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erv348
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 66
|j 2015
|e 20
|b 01
|c 10
|h 6399-413
|