S-genotype identification based on allele-specific PCR in Japanese pear

S-genotype identification based on allele-specific PCR in Japanese pear

Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is n...

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Veröffentlicht in:Breeding science. - 1998. - 65(2015), 3 vom: 28. Juni, Seite 208-15
1. Verfasser: Nashima, Kenji (VerfasserIn)
Weitere Verfasser: Terakami, Shingo, Nishio, Sogo, Kunihisa, Miyuki, Nishitani, Chikako, Saito, Toshihiro, Yamamoto, Toshiya
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Pyrus pyrifolia S-RNase S-genotyping self-incompatibility
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520 |a Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S (1)-S (9) and S (k)) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S (1)/S (1)-S (9)/S (9) and S (4sm)/S (4sm)) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs 
650 4 |a Journal Article 
650 4 |a Pyrus pyrifolia 
650 4 |a S-RNase 
650 4 |a S-genotyping 
650 4 |a self-incompatibility 
700 1 |a Terakami, Shingo  |e verfasserin  |4 aut 
700 1 |a Nishio, Sogo  |e verfasserin  |4 aut 
700 1 |a Kunihisa, Miyuki  |e verfasserin  |4 aut 
700 1 |a Nishitani, Chikako  |e verfasserin  |4 aut 
700 1 |a Saito, Toshihiro  |e verfasserin  |4 aut 
700 1 |a Yamamoto, Toshiya  |e verfasserin  |4 aut 
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773 1 8 |g volume:65  |g year:2015  |g number:3  |g day:28  |g month:06  |g pages:208-15 
856 4 0 |u http://dx.doi.org/10.1270/jsbbs.65.208  |3 Volltext 
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