Standards for plant synthetic biology : a common syntax for exchange of DNA parts
© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
Veröffentlicht in: | The New phytologist. - 1979. - 208(2015), 1 vom: 12. Okt., Seite 13-9 |
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Format: | Online-Aufsatz |
Sprache: | English |
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2015
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Zugriff auf das übergeordnete Werk: | The New phytologist |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. DNA assembly GoldenGate Type IIS restriction endonucleases cloning genetic syntax synthetic biology DNA mehr... |
Zusammenfassung: | © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering |
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Beschreibung: | Date Completed 01.06.2016 Date Revised 07.01.2023 published: Print-Electronic ErratumIn: New Phytol. 2016 Jan;209(2):885. - PMID 26763681 Citation Status MEDLINE |
ISSN: | 1469-8137 |
DOI: | 10.1111/nph.13532 |