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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b01549
|2 doi
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|a pubmed24n0835.xml
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|a (DE-627)NLM250755793
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|a (NLM)26161584
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a van Zalinge, Harm
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Surface-Controlled Properties of Myosin Studied by Electric Field Modulation
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 27.04.2016
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|a Date Revised 04.08.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The efficiency of dynamic nanodevices using surface-immobilized protein molecular motors, which have been proposed for diagnostics, drug discovery, and biocomputation, critically depends on the ability to precisely control the motion of motor-propelled, individual cytoskeletal filaments transporting cargo to designated locations. The efficiency of these devices also critically depends on the proper function of the propelling motors, which is controlled by their interaction with the surfaces they are immobilized on. Here we use a microfluidic device to study how the motion of the motile elements, i.e., actin filaments propelled by heavy mero-myosin (HMM) motor fragments immobilized on various surfaces, is altered by the application of electrical loads generated by an external electric field with strengths ranging from 0 to 8 kVm(-1). Because the motility is intimately linked to the function of surface-immobilized motors, the study also showed how the adsorption properties of HMM on various surfaces, such as nitrocellulose (NC), trimethylclorosilane (TMCS), poly(methyl methacrylate) (PMMA), poly(tert-butyl methacrylate) (PtBMA), and poly(butyl methacrylate) (PBMA), can be characterized using an external field. It was found that at an electric field of 5 kVm(-1) the force exerted on the filaments is sufficient to overcome the frictionlike resistive force of the inactive motors. It was also found that the effect of assisting electric fields on the relative increase in the sliding velocity was markedly higher for the TMCS-derivatized surface than for all other polymer-based surfaces. An explanation of this behavior, based on the molecular rigidity of the TMCS-on-glass surfaces as opposed to the flexibility of the polymer-based ones, is considered. To this end, the proposed microfluidic device could be used to select appropriate surfaces for future lab-on-a-chip applications as illustrated here for the almost ideal TMCS surface. Furthermore, the proposed methodology can be used to gain fundamental insights into the functioning of protein molecular motors, such as the force exerted by the motors under different operational conditions
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Myosins
|2 NLM
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| 650 |
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|a EC 3.6.4.1
|2 NLM
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| 700 |
1 |
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|a Ramsey, Laurence C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aveyard, Jenny
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Persson, Malin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mansson, Alf
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nicolau, Dan V
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 30 vom: 04. Aug., Seite 8354-61
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:31
|g year:2015
|g number:30
|g day:04
|g month:08
|g pages:8354-61
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| 856 |
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|u http://dx.doi.org/10.1021/acs.langmuir.5b01549
|3 Volltext
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|d 31
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|h 8354-61
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