A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost
Long mate pair (LMP) or "jump" libraries are invaluable for producing contiguous genome assemblies and assessing structural variation. However the consistent production of high quality (low duplication rate, accurately sized) LMP libraries has proven problematic in many genome projects. In...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1988. - 59(2015), 1 vom: 22. Juli, Seite 42-5
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1. Verfasser: |
Heavens, Darren
(VerfasserIn) |
Weitere Verfasser: |
Accinelli, Gonzalo Garcia,
Clavijo, Bernardo,
Clark, Matthew Derek |
Format: | Online-Aufsatz
|
Sprache: | English |
Veröffentlicht: |
2015
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Zugriff auf das übergeordnete Werk: | BioTechniques
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Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
genome assembly
insert sizes
long mate pair (LMP) construction
wheat
DNA, Plant |