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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b01928
|2 doi
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|a pubmed24n0835.xml
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|a (DE-627)NLM250673657
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|a (NLM)26153025
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Khaldi, K
|e verfasserin
|4 aut
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|a Active Acetylcholinesterase Immobilization on a Functionalized Silicon Surface
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.04.2016
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|a Date Revised 04.08.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this work, we studied the attachment of active acetylcholinesterase (AChE) enzyme on a silicon substrate as a potential biomarker for the detection of organophosphorous (OP) pesticides. A multistep functionalization strategy was developed on a crystalline silicon surface: a carboxylic acid-terminated monolayer was grafted onto a hydrogen-terminated silicon surface by photochemical hydrosilylation, and then AChE was covalently attached through amide bonds using an activation EDC/NHS process. Each step of the modification was quantitatively characterized by ex-situ Fourier transform infrared spectroscopy in attenuated-total-reflection geometry (ATR-FTIR) and atomic force microscopy (AFM). The kinetics of enzyme immobilization was investigated using in situ real-time infrared spectroscopy. The enzymatic activity of immobilized acetylcholinesterase enzymes was determined with a colorimetric test. The surface concentration of active AChE was estimated to be Γ = 1.72 × 10(10) cm(-2)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biomarkers
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|a Enzymes, Immobilized
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|a Organophosphorus Compounds
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|a Pesticide Residues
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|a Acetylcholinesterase
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|a EC 3.1.1.7
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|a Silicon
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|a Z4152N8IUI
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|a Sam, S
|e verfasserin
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|a Gouget-Laemmel, A C
|e verfasserin
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|a Henry de Villeneuve, C
|e verfasserin
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|a Moraillon, A
|e verfasserin
|4 aut
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|a Ozanam, F
|e verfasserin
|4 aut
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|a Yang, J
|e verfasserin
|4 aut
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|a Kermad, A
|e verfasserin
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|a Ghellai, N
|e verfasserin
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|a Gabouze, N
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 30 vom: 04. Aug., Seite 8421-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:31
|g year:2015
|g number:30
|g day:04
|g month:08
|g pages:8421-8
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|u http://dx.doi.org/10.1021/acs.langmuir.5b01928
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