Live small-animal X-ray lung velocimetry and lung micro-tomography at the Australian Synchrotron Imaging and Medical Beamline

The high flux and coherence produced at long synchrotron beamlines makes them well suited to performing phase-contrast X-ray imaging of the airways and lungs of live small animals. Here, findings of the first live-animal imaging on the Imaging and Medical Beamline (IMBL) at the Australian Synchrotro...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 22(2015), 4 vom: 02. Juli, Seite 1049-55
1. Verfasser: Murrie, Rhiannon P (VerfasserIn)
Weitere Verfasser: Morgan, Kaye S, Maksimenko, Anton, Fouras, Andreas, Paganin, David M, Hall, Chris, Siu, Karen K W, Parsons, David W, Donnelley, Martin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't X-ray lung microtomography phase contrast velocimetry
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520 |a The high flux and coherence produced at long synchrotron beamlines makes them well suited to performing phase-contrast X-ray imaging of the airways and lungs of live small animals. Here, findings of the first live-animal imaging on the Imaging and Medical Beamline (IMBL) at the Australian Synchrotron are reported, demonstrating the feasibility of performing dynamic lung motion measurement and high-resolution micro-tomography. Live anaesthetized mice were imaged using 30 keV monochromatic X-rays at a range of sample-to-detector propagation distances. A frame rate of 100 frames s(-1) allowed lung motion to be determined using X-ray velocimetry. A separate group of humanely killed mice and rats were imaged by computed tomography at high resolution. Images were reconstructed and rendered to demonstrate the capacity for detailed, user-directed display of relevant respiratory anatomy. The ability to perform X-ray velocimetry on live mice at the IMBL was successfully demonstrated. High-quality renderings of the head and lungs visualized both large structures and fine details of the nasal and respiratory anatomy. The effect of sample-to-detector propagation distance on contrast and resolution was also investigated, demonstrating that soft tissue contrast increases, and resolution decreases, with increasing propagation distance. This new capability to perform live-animal imaging and high-resolution micro-tomography at the IMBL enhances the capability for investigation of respiratory diseases and the acceleration of treatment development in Australia 
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650 4 |a X-ray 
650 4 |a lung 
650 4 |a microtomography 
650 4 |a phase contrast 
650 4 |a velocimetry 
700 1 |a Morgan, Kaye S  |e verfasserin  |4 aut 
700 1 |a Maksimenko, Anton  |e verfasserin  |4 aut 
700 1 |a Fouras, Andreas  |e verfasserin  |4 aut 
700 1 |a Paganin, David M  |e verfasserin  |4 aut 
700 1 |a Hall, Chris  |e verfasserin  |4 aut 
700 1 |a Siu, Karen K W  |e verfasserin  |4 aut 
700 1 |a Parsons, David W  |e verfasserin  |4 aut 
700 1 |a Donnelley, Martin  |e verfasserin  |4 aut 
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