The Casuarina NIN gene is transcriptionally activated throughout Frankia root infection as well as in response to bacterial diffusible signals

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 208(2015), 3 vom: 01. Nov., Seite 887-903
1. Verfasser:	Clavijo, Fernando (VerfasserIn)
Weitere Verfasser:	Diedhiou, Issa, Vaissayre, Virginie, Brottier, Laurent, Acolatse, Jennifer, Moukouanga, Daniel, Crabos, Amandine, Auguy, Florence, Franche, Claudine, Gherbi, Hassen, Champion, Antony, Hocher, Valerie, Barker, David, Bogusz, Didier, Tisa, Louis S, Svistoonoff, Sergio
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. actinorhiza biological nitrogen fixation mycorrhiza nodule inception plant-microbe signalling root hair deformation root nodule symbioses mehr... NIN protein, Lotus japonicus Plant Proteins

Beschreibung
Zusammenfassung:	© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Root nodule symbioses (RNS) allow plants to acquire atmospheric nitrogen by establishing an intimate relationship with either rhizobia, the symbionts of legumes or Frankia in the case of actinorhizal plants. In legumes, NIN (Nodule INception) genes encode key transcription factors involved in nodulation. Here we report the characterization of CgNIN, a NIN gene from the actinorhizal tree Casuarina glauca using both phylogenetic analysis and transgenic plants expressing either ProCgNIN::reporter gene fusions or CgNIN RNAi constructs. We have found that CgNIN belongs to the same phylogenetic group as other symbiotic NIN genes and CgNIN is able to complement a legume nin mutant for the early steps of nodule development. CgNIN expression is correlated with infection by Frankia, including preinfection stages in developing root hairs, and is induced by culture supernatants. Knockdown mutants were impaired for nodulation and early root hair deformation responses were severely affected. However, no mycorrhizal phenotype was observed and no induction of CgNIN expression was detected in mycorrhizas. Our results indicate that elements specifically required for nodulation include NIN and possibly related gene networks derived from the nitrate signalling pathways
Beschreibung:	Date Completed 15.08.2016 Date Revised 30.09.2020 published: Print-Electronic GENBANK: KF481969 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.13506