The Arabidopsis mitogen-activated protein kinase 6 is associated with γ-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 207(2015), 4 vom: 08. Sept., Seite 1061-74
1. Verfasser:	Kohoutová, Lucie (VerfasserIn)
Weitere Verfasser:	Kourová, Hana, Nagy, Szilvia K, Volc, Jindřich, Halada, Petr, Mészáros, Tamás, Meskiene, Irute, Bögre, László, Binarová, Pavla
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis EB1c MPK6 cell division microtubules mitogen-activated protein kinase nitrosative stress γ-tubulin mehr... Arabidopsis Proteins Butadienes EB1c protein, Arabidopsis Microtubule-Associated Proteins Nitriles Tubulin U 0126 3-nitrotyrosine 3604-79-3 Tyrosine 42HK56048U Extracellular Signal-Regulated MAP Kinases EC 2.7.11.24 MPK6 protein, Arabidopsis Mitogen-Activated Protein Kinases

Beschreibung
Zusammenfassung:	© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Stress-activated plant mitogen-activated protein (MAP) kinase pathways play roles in growth adaptation to the environment by modulating cell division through cytoskeletal regulation, but the mechanisms are poorly understood. We performed protein interaction and phosphorylation experiments with cytoskeletal proteins, mass spectrometric identification of MPK6 complexes and immunofluorescence analyses of the microtubular cytoskeleton of mitotic cells using wild-type, mpk6-2 mutant and plants overexpressing the MAP kinase-inactivating phosphatase, AP2C3. We showed that MPK6 interacted with γ-tubulin and co-sedimented with plant microtubules polymerized in vitro. It was the active form of MAP kinase that was enriched with microtubules and followed similar dynamics to γ-tubulin, moving from poles to midzone during the anaphase-to-telophase transition. We found a novel substrate for MPK6, the microtubule plus end protein, EB1c. The mpk6-2 mutant was sensitive to 3-nitro-l-tyrosine (NO2 -Tyr) treatment with respect to mitotic abnormalities, and root cells overexpressing AP2C3 showed defects in chromosome segregation and spindle orientation. Our data suggest that the active form of MAP kinase interacts with γ-tubulin on specific subsets of mitotic microtubules during late mitosis. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions
Beschreibung:	Date Completed 04.05.2016 Date Revised 18.03.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.13501