Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries
Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection-based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (ssDNA) fluorescent probes through an intermed...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 58(2015), 6 vom: 13. Juni, Seite 301-7
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| 1. Verfasser: |
Murgha, Yusuf
(VerfasserIn) |
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| Weitere Verfasser: |
Beliveau, Brian,
Semrau, Kassandra,
Schwartz, Donald,
Wu, Chao-Ting,
Gulari, Erdogan,
Rouillard, Jean-Marie |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Oligopaint FISH probes
fluorescent in situ hybridization (FISH)
in vitro transcription (IVT)
microarrays
oligonucleotide library pools
reverse transcription (RT)
single-stranded DNA (ssDNA) libraries
mehr...
DNA Primers
DNA, Single-Stranded
Fluorescent Dyes
Oligonucleotide Probes
RNA
63231-63-0 |
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