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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b00993
|2 doi
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|a pubmed24n0832.xml
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|a (DE-627)NLM249702568
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|a (NLM)26048043
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Majzoub, Ramsey N
|e verfasserin
|4 aut
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| 245 |
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|a Patterned Threadlike Micelles and DNA-Tethered Nanoparticles
|b A Structural Study of PEGylated Cationic Liposome-DNA Assemblies
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 24.03.2016
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|a Date Revised 03.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The self-assembly of oppositely charged biomacromolecules has been extensively studied due to its pertinence in the design of functional nanomaterials. Using cryo electron microscopy (cryo-EM), optical light scattering, and fluorescence microscopy, we investigated the structure and phase behavior of PEGylated (PEG: poly(ethylene glycol)) cationic liposome-DNA nanoparticles (CL-DNA NPs) as a function of DNA length, topology (linear and circular), and ρ(chg) (the molar charge ratio of cationic lipid to anionic DNA). Although all NPs studied exhibited lamellar internal nanostructure, NPs formed with short (∼2 kbps), linear, polydisperse DNA were defect-rich and contained smaller domains. Unexpectedly, we found distinctly different equilibrium structures away from the isoelectric point. At ρ(chg) > 1, in the excess cationic lipid regime, threadlike micelles rich in PEG-lipid were found to coexist with NPs, cationic liposomes, and spherical micelles. At high concentrations these PEGylated threadlike micelles formed a well-ordered, patterned morphology with highly uniform intermicellar spacing. At ρ(chg) < 1, in the excess DNA regime and with no added salt, individual NPs were tethered together via long, linear DNA (48 kbps λ-phage DNA) into a biopolymer-mediated floc. Our results provide insight into what equilibrium nanostructures can form when oppositely charged macromolecules self-assemble in aqueous media. Self-assembled, well-ordered threadlike micelles and tethered nanoparticles may have a broad range of applications in bionanotechnology, including nanoscale lithograpy and the development of lipid-based multifunctional nanoparticle networks
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Liposomes
|2 NLM
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|a Micelles
|2 NLM
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|a Polyethylene Glycols
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|a 3WJQ0SDW1A
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|a DNA
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|a 9007-49-2
|2 NLM
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|a Ewert, Kai K
|e verfasserin
|4 aut
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|a Jacovetty, Erica L
|e verfasserin
|4 aut
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|a Carragher, Bridget
|e verfasserin
|4 aut
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|a Potter, Clinton S
|e verfasserin
|4 aut
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|a Li, Youli
|e verfasserin
|4 aut
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|a Safinya, Cyrus R
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 25 vom: 30. Juni, Seite 7073-83
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:31
|g year:2015
|g number:25
|g day:30
|g month:06
|g pages:7073-83
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|u http://dx.doi.org/10.1021/acs.langmuir.5b00993
|3 Volltext
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