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NLM249671913 |
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DE-627 |
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20231224154107.0 |
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cr uuu---uuuuu |
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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-015-1502-0
|2 doi
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2 |
|a pubmed24n0832.xml
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|a (DE-627)NLM249671913
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|a (NLM)26044931
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Li-Ying
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Cloning of the Aegiceras corniculatum class I chitinase gene (AcCHI I) and the response of AcCHI I mRNA expression to cadmium stress
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| 264 |
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1 |
|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 31.08.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Chitinases in terrestrial plants have been reported these are involved in heavy metal tolerance/detoxification. This is the first attempt to reveal chitinase gene (AcCHI I) and its function on metal detoxification in mangroves Aegiceras corniculatum. RT-PCR and RACE techniques were used to clone AcCHI I, while real-time quantitative PCR was employed to assess AcCHI I mRNA expressions in response to Cadmium (Cd). The deduced AcCHI I protein consists of 316 amino acids, including a signal peptide region, a chitin-binding domain (CBD) and a catalytic domain. Protein homology modeling was performed to identify potential features in AcCHI I. The CBD structure of AcCHI I might be critical for metal tolerance/homeostasis of the plant. Clear tissue-specific differences in AcCHI I expression were detected, with higher transcript levels detected in leaves. Results demonstrated that a short duration of Cd exposure (e.g., 3 days) promoted AcCHI I expression in roots. Upregulated expression was also detected in leaves under 10 mg/kg Cd concentration stress. The present study demonstrates that AcCHI I may play an important role in Cd tolerance/homeostasis in the plant. Further studies of the AcCHI I protein, gene overexpression, the promoter and upstream regulation will be necessary for clarifying the functions of AcCHI I
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Aegiceras corniculatum
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| 650 |
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4 |
|a Cd
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| 650 |
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|a Chitinase
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| 650 |
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4 |
|a Cloning
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| 650 |
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4 |
|a Expression
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| 650 |
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4 |
|a Mangrove plant
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a Water Pollutants, Chemical
|2 NLM
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| 650 |
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7 |
|a Cadmium
|2 NLM
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| 650 |
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7 |
|a 00BH33GNGH
|2 NLM
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| 650 |
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7 |
|a Chitinases
|2 NLM
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| 650 |
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7 |
|a EC 3.2.1.14
|2 NLM
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| 650 |
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7 |
|a chitinase C
|2 NLM
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| 650 |
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7 |
|a EC 3.2.1.14
|2 NLM
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| 700 |
1 |
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|a Wang, You-Shao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jing-Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yeok, Foong Swee
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 24(2015), 7-8 vom: 05. Okt., Seite 1705-13
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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| 773 |
1 |
8 |
|g volume:24
|g year:2015
|g number:7-8
|g day:05
|g month:10
|g pages:1705-13
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| 856 |
4 |
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|u http://dx.doi.org/10.1007/s10646-015-1502-0
|3 Volltext
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|a GBV_ILN_65
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|a GBV_ILN_350
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|a AR
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|d 24
|j 2015
|e 7-8
|b 05
|c 10
|h 1705-13
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