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DE-627 |
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20231224153134.0 |
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cr uuu---uuuuu |
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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1080/08997659.2014.993484
|2 doi
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|a pubmed24n0830.xml
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|a (DE-627)NLM249237814
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|a (NLM)25997556
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Hu, Kun
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Chitosan Influences the Expression of P-gp and Metabolism of Norfloxacin in Grass Carp
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| 264 |
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 18.09.2015
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a The aim of this study was to investigate the relationship between the administration of chitosan (CTS), expression of permeability glycoprotein (P-gp), and the metabolism of norfloxacin (NOR) in Grass Carp Ctenopharyngodon idella. Fish were administrated with a single dose of either NOR, CTS, 1:5 NOR-CTS or 1:10 NOR-CTS. The P-gp expression was analyzed by immunohistochemistry and real time-PCR. The concentration of NOR was determined using HPLC. The mRNA and protein expression of P-gp in the fish intestine was significantly enhanced following a single dosage of 40 mg/kg NOR, and peak expression occurred at 3 h after drug administration (P < 0.05). A single dosage of both 1:5 NOR-CTS and 1:10 NOR-CTS reduced the intestinal P-gp expression to levels significantly lower than that from NOR alone (P < 0.05), but significantly higher than that from the control (P < 0.05). Interestingly, CTS alone also led to a slight decrease in P-gp expression. In addition, pharmacokinetic assays revealed a marked increase in area under the curve (AUC) of NOR with 1:5 and 1:10 NOR-CTS, by approximately 1.5-fold and threefold, respectively. Finally, the relative bioavailability of NOR after a single oral dosage of 1:5 and 1:10 NOR-CTS was enhanced to 148.02% and 304.98%, respectively. In this study, we demonstrated that the transmembrane glycoprotein P-gp regulates NOR metabolism in the intestine of Grass Carp, suggesting that NOR may be a direct substrate of P-gp. More importantly, we showed that CTS can inhibit P-gp expression in a dose-dependent manner and improve the relative bioavailability of NOR in this species
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a ATP Binding Cassette Transporter, Subfamily B, Member 1
|2 NLM
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|a Anti-Bacterial Agents
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Chitosan
|2 NLM
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| 650 |
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|a 9012-76-4
|2 NLM
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| 650 |
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|a Norfloxacin
|2 NLM
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| 650 |
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|a N0F8P22L1P
|2 NLM
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| 700 |
1 |
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|a Xie, Xinyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Yi-Ni
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ruan, Jiming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Hao-Ran
|e verfasserin
|4 aut
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| 700 |
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|a Jin, Tianyi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Xian-Le
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 27(2015), 2 vom: 22. Juni, Seite 104-11
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnns
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| 773 |
1 |
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|g volume:27
|g year:2015
|g number:2
|g day:22
|g month:06
|g pages:104-11
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| 856 |
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|u http://dx.doi.org/10.1080/08997659.2014.993484
|3 Volltext
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