Non-destructive high-throughput DNA extraction and genotyping methods for cotton seeds and seedlings
Extensive use of targeted PCR-based genotyping is precluded for many plant research laboratories by the cost and time required for DNA extraction. Using cotton (Gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-th...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 58(2015), 5 vom: 13. Mai, Seite 234-43
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| 1. Verfasser: |
Zheng, Xiuting
(VerfasserIn) |
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| Weitere Verfasser: |
Hoegenauer, Kevin A,
Maeda, Andrea B V,
Wang, Fei,
Stelly, David M,
Nichols, Robert L,
Jones, Don C |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA extraction
cotton
cotyledon
genotyping
seed
seedling
DNA, Plant |
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