|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM24884363X |
| 003 |
DE-627 |
| 005 |
20231224152300.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erv169
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0829.xml
|
| 035 |
|
|
|a (DE-627)NLM24884363X
|
| 035 |
|
|
|a (NLM)25956880
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Li, Qian
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.03.2016
|
| 500 |
|
|
|a Date Revised 10.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Sweet cherry is a non-climacteric fruit and its ripening is regulated by abscisic acid (ABA) during fruit development. In this study, four cDNAs (PacCYP707A1-4) encoding 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA, were identified in sweet cherry fruits using tobacco rattle virus-induced gene silencing (VIGS) and particle bombardment approaches. Quantitative real-time PCR confirmed significant down-regulation of target gene transcripts in VIGS-treated cherry fruits. In PacCYP707A2-RNAi-treated fruits, ripening and fruit colouring were promoted relative to control fruits, and both ABA accumulation and PacNCED1 transcript levels were up-regulated by 140%. Silencing of PacCYP707A2 by VIGS significantly altered the transcripts of both ABA-responsive and ripening-related genes, including the ABA metabolism-associated genes NCED and CYP707A, the anthocyanin synthesis genes PacCHS, PacCHI, PacF3H, PacDFR, PacANS, and PacUFGT, the ethylene biosynthesis gene PacACO1, and the transcription factor PacMYBA. The promoter of PacMYBA responded more strongly to PacCYP707A2-RNAi-treated fruits than to PacCYP707A1-RNAi-treated fruits. By contrast, silencing of PacCYP707A1 stimulated a slight increase in fruit colouring and enhanced resistance to dehydration stress compared with control fruits. These results suggest that PacCYP707A2 is a key regulator of ABA catabolism that functions as a negative regulator of fruit ripening, while PacCYP707A1 regulates ABA content in response to dehydration during fruit development
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a ABA
|
| 650 |
|
4 |
|a CYP707A
|
| 650 |
|
4 |
|a VIGS.
|
| 650 |
|
4 |
|a cherry ripening
|
| 650 |
|
4 |
|a colouring
|
| 650 |
|
4 |
|a dehydration stress
|
| 650 |
|
4 |
|a particle bombardment
|
| 650 |
|
7 |
|a Anthocyanins
|2 NLM
|
| 650 |
|
7 |
|a Ethylenes
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Abscisic Acid
|2 NLM
|
| 650 |
|
7 |
|a 72S9A8J5GW
|2 NLM
|
| 650 |
|
7 |
|a ethylene
|2 NLM
|
| 650 |
|
7 |
|a 91GW059KN7
|2 NLM
|
| 700 |
1 |
|
|a Chen, Pei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dai, Shengjie
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sun, Yufei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yuan, Bing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kai, Wenbin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pei, Yuelin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a He, Suihuan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liang, Bin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Yushu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Leng, Ping
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 66(2015), 13 vom: 08. Juli, Seite 3765-74
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:66
|g year:2015
|g number:13
|g day:08
|g month:07
|g pages:3765-74
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erv169
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 66
|j 2015
|e 13
|b 08
|c 07
|h 3765-74
|