Rab from the white shrimp Litopenaeus vannamei : characterization and its regulation upon environmental stress

With the destruction of the ecological environment, shrimp cultivation in China has been seriously affected by outbreaks of infectious diseases. Rab, which belong to small GTPase Ras superfamily, can regulate multiple steps in eukaryotic vesicle trafficking including vesicle budding, vesicle tetheri...

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Veröffentlicht in:Ecotoxicology (London, England). - 1992. - 24(2015), 7-8 vom: 21. Okt., Seite 1765-74
1. Verfasser: Wang, Lei (VerfasserIn)
Weitere Verfasser: Wang, Xiao-Rong, Liu, Jin, Chen, Chu-Xian, Liu, Yuan, Wang, Wei-Na
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Ecotoxicology (London, England)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Environmental stresses Litopenaeus vannamei Quantitative real-time PCR Rab Arthropod Proteins DNA, Complementary RNA, Messenger ras Proteins EC 3.6.5.2
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520 |a With the destruction of the ecological environment, shrimp cultivation in China has been seriously affected by outbreaks of infectious diseases. Rab, which belong to small GTPase Ras superfamily, can regulate multiple steps in eukaryotic vesicle trafficking including vesicle budding, vesicle tethering, and membrane fusion. Knowledge of Rab in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we analyzed the functions of Rab from the Pacific white shrimp, Litopenaeus vannamei. Full-length cDNA of Rab was obtained, which was 751 bp long, with open reading frame encoding 206 amino acids. In this study, for the first time, the gene expression of Rab of L. vannamei was analyzed by quantitative real-time PCR after exposure to five kinds of environmental stresses (bacteria, pH, Cd, salinity and low temperature). The results demonstrate that Rab is sensitive and involved in bacteria, pH, and Cd stress responses and Rab is more sensitive to bacteria than other stresses. Therefore we infer that Rab may have relationship with the anti-stress mechanism induced by environment stress in shrimp and Rab could be used as critical biomarkers for environmental quality assessment 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Environmental stresses 
650 4 |a Litopenaeus vannamei 
650 4 |a Quantitative real-time PCR 
650 4 |a Rab 
650 7 |a Arthropod Proteins  |2 NLM 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a ras Proteins  |2 NLM 
650 7 |a EC 3.6.5.2  |2 NLM 
700 1 |a Wang, Xiao-Rong  |e verfasserin  |4 aut 
700 1 |a Liu, Jin  |e verfasserin  |4 aut 
700 1 |a Chen, Chu-Xian  |e verfasserin  |4 aut 
700 1 |a Liu, Yuan  |e verfasserin  |4 aut 
700 1 |a Wang, Wei-Na  |e verfasserin  |4 aut 
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