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|a 10.1093/jxb/erv207
|2 doi
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|a eng
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|a Na, Jong-Kuk
|e verfasserin
|4 aut
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|a Expression of potato RNA-binding proteins StUBA2a/b and StUBA2c induces hypersensitive-like cell death and early leaf senescence in Arabidopsis
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.03.2016
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a The Arabidopsis thaliana genome encodes three RNA-binding proteins (RBPs), UBP1-associated protein 2a (UBA2a), UBA2b, and UBA2c, that contain two RNA-recognition motif (RRM) domains. They play important roles in wounding response and leaf senescence, and are homologs of Vicia faba abscisic-acid-activated protein kinase-interacting protein 1 (VfAKIP1). The potato (Solanum tuberosum) genome encodes at least seven AKIP1-like RBPs. Here, two potato RBPs have been characterized, StUBA2a/b and StUBA2c, that are homologous to VfAKIP1 and Arabidopsis UBA2s. Transient expression of StUBA2s induced a hypersensitive-like cell death phenotype in tobacco leaves, and an RRM-domain deletion assay of StUBA2s revealed that the first RRM domain is crucial for the phenotype. Unlike overexpression of Arabidopsis UBA2s, constitutive expression of StUBA2a/b in Arabidopsis did not cause growth arrest and lethality at the young seedling stage, but induced early leaf senescence. This phenotype was associated with increased expression of defence- and senescence-associated genes, including pathogen-related genes (PR) and a senescence-associated gene (SAG13), and it was aggravated upon flowering and ultimately resulted in a shortened life cycle. Leaf senescence of StUBA2a/b Arabidopsis plants was enhanced under darkness and was accompanied by H2O2 accumulation and altered expression of autophagy-associated genes, which likely cause cellular damage and are proximate causes of the early leaf senescence. Expression of salicylic acid signalling and biosynthetic genes was also upregulated in StUBA2a/b plants. Consistent with the localization of UBA2s-GFPs and VfAKIP1-GFP, soluble-modified GFP-StUBA2s localized in the nucleus within nuclear speckles. StUBA2s potentially can be considered for transgenic approaches to induce potato shoot senescence, which is desirable at harvest
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a AKIP proteins
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|a Arabidopsis thaliana
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|a RNA binding proteins
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|a Solanum tuberosum
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|a UBA2 proteins.
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|a senescence
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|a Plant Proteins
|2 NLM
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|a RNA-Binding Proteins
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a Hydrogen Peroxide
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|a BBX060AN9V
|2 NLM
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| 700 |
1 |
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|a Kim, Jae-Kwang
|e verfasserin
|4 aut
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|a Kim, Dool-Yi
|e verfasserin
|4 aut
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| 700 |
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|a Assmann, Sarah M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 66(2015), 13 vom: 05. Juli, Seite 4023-33
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:66
|g year:2015
|g number:13
|g day:05
|g month:07
|g pages:4023-33
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|u http://dx.doi.org/10.1093/jxb/erv207
|3 Volltext
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