OsAGSW1, an ABC1-like kinase gene, is involved in the regulation of grain size and weight in rice

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 66(2015), 19 vom: 15. Sept., Seite 5691-701
1. Verfasser: Li, Tao (VerfasserIn)
Weitere Verfasser: Jiang, Jieming, Zhang, Shengchun, Shu, Haoran, Wang, Yaqin, Lai, Jianbin, Du, Jinju, Yang, Chengwei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ABC1-like kinase OsAGSW1. grain development grain size grain weight grain width Plant Proteins
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520 |a Grain shape and weight are two determining agronomic traits of rice yield. ABC1 (Activity of bc1 complex) is a newly found atypical kinase in plants. Here, we report on an ABC1 protein kinase gene, OsAGSW1 (ABC1-like kinase related to Grain size and Weight). Expression of OsAGSW1-GFP in rice revealed that OsAGSW1 is localized to the chloroplasts in rice. Analysis of OsAGSW1 promoter::β-glucuronidase transgenic rice indicated that this gene was highly expressed in vascular bundles in shoot, hull and caryopsis. Furthermore, OsAGSW1-RNAi and overexpressed transgenic rice lines were generated. Stable transgenic lines overexpressing OsAGSW1 exhibited a phenotype with a significant increase in grain size, grain weight, grain filling rate and 1000-grain weight compared with the wild-type and RNAi transgenic plants. Microscopy analysis showed that spikelet hulls just before heading were different in the OsAGSW1-overexpressed plants compared with wild-type and OsAGSW1 RNAi rice. Further cytological analysis showed that the number of external parenchyma cells in rice hulls of OsAGSW1-overexpressed plants increased, leading to wider and longer spikelet hulls than those of the wild-type and OsAGSW1-RNAi plants. The vascular cross-sectional area in lemma, carpopodium and ovules also strikingly increased and area of both xylem and phloem were enlarged in the OsAGSW1-overexpressed plants. Thus, our results demonstrated that OsAGSW1 plays an important role in seed shape and size of rice by regulating the number of external parenchyma cells and the development of vascular bundles, providing a new insight into the functions of ABC1 genes in plants 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a ABC1-like kinase 
650 4 |a OsAGSW1. 
650 4 |a grain development 
650 4 |a grain size 
650 4 |a grain weight 
650 4 |a grain width 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Jiang, Jieming  |e verfasserin  |4 aut 
700 1 |a Zhang, Shengchun  |e verfasserin  |4 aut 
700 1 |a Shu, Haoran  |e verfasserin  |4 aut 
700 1 |a Wang, Yaqin  |e verfasserin  |4 aut 
700 1 |a Lai, Jianbin  |e verfasserin  |4 aut 
700 1 |a Du, Jinju  |e verfasserin  |4 aut 
700 1 |a Yang, Chengwei  |e verfasserin  |4 aut 
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