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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2015.04.002
|2 doi
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|a pubmed25n0828.xml
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|a (DE-627)NLM24840573X
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|a (NLM)25910650
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|a (PII)S0981-9428(15)30003-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hung, Chun-Hsien
|e verfasserin
|4 aut
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|a Isolation and characterization of a phosphatidylglycerophosphate phosphatase1, PGPP1, in Chlamydomonas reinhardtii
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.02.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Masson SAS. All rights reserved.
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|a Phosphatidylglycerol (PG) is the exclusive phospholipid synthesized in chloroplasts and plays important roles in photosynthesis. However, phosphatidylglycerophosphate phosphatase (PGPP), which catalyzes the final step of PG biosynthesis, is a missing piece in photosynthetic eukaryotes. Here, we isolated a previously uncharacterized haloacid dehalogenase-like phosphatase, designated CrPGPP1, as a putative PGPP in Chlamydomonas reinhardtii. CrPGPP1 complemented growth and lipid compositional defects in Δgep4, a yeast mutant of PGPP, which indicates that CrPGPP1 is a functional PGPP. Two aspartic acid residues, which are both essential for the yeast PGPP (Gep4p) activity, are also conserved in the putative catalytic motif of CrPGPP1. Site-specific mutagenesis showed that the first but not the second aspartic acid residue was required for CrPGPP1 to complement the growth defect of Δgep4 mutant, which highlights the distinct molecular features of CrPGPP1. Our results suggest that CrPGPP1 is a functional PGPP in C. reinhardtii, for the first PGPP in photosynthetic eukaryotes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Chlamydomonas reinhardtii
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|a Chloroplast
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|a Phosphatidylglycerol
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|a Phosphatidylglycerophosphate phosphatase
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|a Photosynthesis
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|a Phosphatidylglycerols
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a phosphatidylglycerophosphate
|2 NLM
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|a Aspartic Acid
|2 NLM
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|a 30KYC7MIAI
|2 NLM
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| 650 |
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|a Phosphoric Monoester Hydrolases
|2 NLM
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| 650 |
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|a EC 3.1.3.2
|2 NLM
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| 700 |
1 |
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|a Kobayashi, Koichi
|e verfasserin
|4 aut
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|a Wada, Hajime
|e verfasserin
|4 aut
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| 700 |
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|a Nakamura, Yuki
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 92(2015) vom: 24. Juli, Seite 56-61
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:92
|g year:2015
|g day:24
|g month:07
|g pages:56-61
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|u http://dx.doi.org/10.1016/j.plaphy.2015.04.002
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