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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b00443
|2 doi
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|a pubmed24n0828.xml
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|a (DE-627)NLM248397907
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|a (NLM)25909861
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Polak, Roberta
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Optimization of amine-rich multilayer thin films for the capture and quantification of prostate-specific antigen
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.02.2016
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|a Date Revised 19.05.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Biomarkers, Tumor
|2 NLM
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| 650 |
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|a Polyamines
|2 NLM
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|a Polystyrenes
|2 NLM
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|a polyallylamine
|2 NLM
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|a 30551-89-4
|2 NLM
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|a polystyrene sulfonic acid
|2 NLM
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|a 70KO0R01RY
|2 NLM
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| 650 |
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|a Prostate-Specific Antigen
|2 NLM
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| 650 |
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|a EC 3.4.21.77
|2 NLM
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| 700 |
1 |
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|a Bradwell, Grinia M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gilbert, Jonathan B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Danielsen, Scott
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Beppu, Marisa M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cohen, Robert E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rubner, Michael F
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 19 vom: 19. Mai, Seite 5479-88
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:31
|g year:2015
|g number:19
|g day:19
|g month:05
|g pages:5479-88
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|u http://dx.doi.org/10.1021/acs.langmuir.5b00443
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