How cell wall complexity influences saccharification efficiency in Miscanthus sinensis

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 66(2015), 14 vom: 23. Juli, Seite 4351-65
1. Verfasser: De Souza, Amanda P (VerfasserIn)
Weitere Verfasser: Alvim Kamei, Claire L, Torres, Andres F, Pattathil, Sivakumar, Hahn, Michael G, Trindade, Luisa M, Buckeridge, Marcos S
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Bioenergy cell wall polysaccharides lignin recalcitrance saccharification.
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520 |a The production of bioenergy from grasses has been developing quickly during the last decade, with Miscanthus being among the most important choices for production of bioethanol. However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure. Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures. In this work, a set of different techniques was used to access the complexity of cell walls of different genotypes of Miscanthus sinensis in order to understand how they interfere with saccharification efficiency. Three genotypes of M. sinensis displaying different patterns of correlation between lignin content and saccharification efficiency were subjected to cell wall analysis by quantitative/qualitative analytical techniques such as monosaccharide composition, oligosaccharide profiling, and glycome profiling. When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis. In the absence of such correlation, different types of pectins, and some mannans contributed to saccharification efficiency. Different genotypes of M. sinensis were shown to display distinct interactions among their cell wall components, which seem to influence cell wall hydrolysis 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Bioenergy 
650 4 |a cell wall polysaccharides 
650 4 |a lignin 
650 4 |a recalcitrance 
650 4 |a saccharification. 
700 1 |a Alvim Kamei, Claire L  |e verfasserin  |4 aut 
700 1 |a Torres, Andres F  |e verfasserin  |4 aut 
700 1 |a Pattathil, Sivakumar  |e verfasserin  |4 aut 
700 1 |a Hahn, Michael G  |e verfasserin  |4 aut 
700 1 |a Trindade, Luisa M  |e verfasserin  |4 aut 
700 1 |a Buckeridge, Marcos S  |e verfasserin  |4 aut 
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