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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2015.03.013
|2 doi
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|a pubmed24n0827.xml
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|a (DE-627)NLM24830299X
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|a (NLM)25899729
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|a (PII)S0176-1617(15)00082-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Uji, Yuya
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Isolation of a sesquiterpene synthase expressing in specialized epithelial cells surrounding the secretory cavities in rough lemon (Citrus jambhiri)
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.02.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier GmbH. All rights reserved.
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|a Volatile terpenoids such as monoterpenes and sesquiterpenes play multiple roles in plant responses and are synthesized by terpene synthases (TPSs). We have previously isolated a partial TPS gene, RlemTPS4, that responds to microbial attack in rough lemon. In this study, we isolated a full length RlemTPS4 cDNA from rough lemon. RlemTPS4 localized in the cytosol. The recombinant RlemTPS4 protein was obtained using a prokaryotic expression system and GC-MS analysis of the terpenes produced by the RlemTPS4 enzymatic reaction determined that RlemTPS4 produces some sesquiterpenes such as δ-elemene. The RlemTPS4 gene was specifically expressed in specialized epithelial cells surrounding the oil secretory cavities in rough lemon leaf tissue
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Citrus
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| 650 |
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|a Essential oil
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| 650 |
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|a Sesquiterpene
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| 650 |
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|a Terpene synthase
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|a δ-Elemene
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Alkyl and Aryl Transferases
|2 NLM
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| 650 |
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|a EC 2.5.-
|2 NLM
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| 650 |
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|a terpene synthase
|2 NLM
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| 650 |
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|a EC 2.5.1.-
|2 NLM
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| 700 |
1 |
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|a Ozawa, Rika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shishido, Hodaka
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Taniguchi, Shiduku
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Takabayashi, Junji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Akimitsu, Kazuya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gomi, Kenji
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 180(2015) vom: 15. Mai, Seite 67-71
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:180
|g year:2015
|g day:15
|g month:05
|g pages:67-71
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2015.03.013
|3 Volltext
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|d 180
|j 2015
|b 15
|c 05
|h 67-71
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