Characterizing visible and invisible cell wall mutant phenotypes

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 66(2015), 14 vom: 14. Juli, Seite 4145-63
1. Verfasser: Carpita, Nicholas C (VerfasserIn)
Weitere Verfasser: McCann, Maureen C
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Review Capillary electrophoresis Fourier transform infrared (FTIR) cell wall mutant cell wall synthesis electrospray ionization (ESI) mass spectrometry (MS) matrix-assisted laser desorption ionization (MALDI) mehr... methylation analysis principal components analysis (PCA).
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520 |a About 10% of a plant's genome is devoted to generating the protein machinery to synthesize, remodel, and deconstruct the cell wall. High-throughput genome sequencing technologies have enabled a reasonably complete inventory of wall-related genes that can be assembled into families of common evolutionary origin. Assigning function to each gene family member has been aided immensely by identification of mutants with visible phenotypes or by chemical and spectroscopic analysis of mutants with 'invisible' phenotypes of modified cell wall composition and architecture that do not otherwise affect plant growth or development. This review connects the inference of gene function on the basis of deviation from the wild type in genetic functional analyses to insights provided by modern analytical techniques that have brought us ever closer to elucidating the sequence structures of the major polysaccharide components of the plant cell wall 
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650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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650 4 |a Capillary electrophoresis 
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650 4 |a electrospray ionization (ESI) 
650 4 |a mass spectrometry (MS) 
650 4 |a matrix-assisted laser desorption ionization (MALDI) 
650 4 |a methylation analysis 
650 4 |a principal components analysis (PCA). 
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