Optimization for extracellular polymeric substances extraction of microbial aggregates

The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods - ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat - were investigated in the study, and the compone...

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Veröffentlicht in:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 71(2015), 7 vom: 11., Seite 1106-12
1. Verfasser: Zhu, Liang (VerfasserIn)
Weitere Verfasser: Yu, Haitian, Liu, Yimei, Qi, Hanying, Xu, Xiangyang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Water science and technology : a journal of the International Association on Water Pollution Research
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cation Exchange Resins Formamides Polymers Sewage Sulfides formamide 4781T907ZS Sodium Hydroxide 55X04QC32I
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520 |a The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods - ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat - were investigated in the study, and the component differences of extracted EPS from the loose flocs and dense aerobic granules were compared using chemical analysis and three-dimensional excitation-emission matrix (3D-EEM). Results showed that the contents of EPS were extracted effectively by ultrasound + formamide + NaOH and ultrasound + heat methods, and the ultrasound + CER + sulfide method did not extract the polysaccharides (PS) or protein (PN) contents from the sludge samples. The 3D-EEM analysis indicated that the nature of peak B/D, peak C/E/F, and peak A/G were attributed to PN-like, humic acid-like and fulvic acid-like fluorophores. All fluorophores can be detected from the EPS extracted through the ultrasound + heat method. Hopefully this will provide more information about the EPS interaction mechanism of microbial aggregates 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Formamides  |2 NLM 
650 7 |a Polymers  |2 NLM 
650 7 |a Sewage  |2 NLM 
650 7 |a Sulfides  |2 NLM 
650 7 |a formamide  |2 NLM 
650 7 |a 4781T907ZS  |2 NLM 
650 7 |a Sodium Hydroxide  |2 NLM 
650 7 |a 55X04QC32I  |2 NLM 
700 1 |a Yu, Haitian  |e verfasserin  |4 aut 
700 1 |a Liu, Yimei  |e verfasserin  |4 aut 
700 1 |a Qi, Hanying  |e verfasserin  |4 aut 
700 1 |a Xu, Xiangyang  |e verfasserin  |4 aut 
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