Oakleaf an S locus-linked mutation of Primula vulgaris that affects leaf and flower development

Oakleaf : an S locus-linked mutation of Primula vulgaris that affects leaf and flower development

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 208(2015), 1 vom: 06. Okt., Seite 149-61
1. Verfasser: Cocker, Jonathan M (VerfasserIn)
Weitere Verfasser: Webster, Margaret A, Li, Jinhong, Wright, Jonathan, Kaithakottil, Gemy, Swarbreck, David, Gilmartin, Philip M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't KNOX genes Oakleaf Primula vulgaris S locus heterostyly DNA, Plant
Beschreibung
Zusammenfassung:© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
In Primula vulgaris outcrossing is promoted through reciprocal herkogamy with insect-mediated cross-pollination between pin and thrum form flowers. Development of heteromorphic flowers is coordinated by genes at the S locus. To underpin construction of a genetic map facilitating isolation of these S locus genes, we have characterised Oakleaf, a novel S locus-linked mutant phenotype. We combine phenotypic observation of flower and leaf development, with classical genetic analysis and next-generation sequencing to address the molecular basis of Oakleaf. Oakleaf is a dominant mutation that affects both leaf and flower development; plants produce distinctive lobed leaves, with occasional ectopic meristems on the veins. This phenotype is reminiscent of overexpression of Class I KNOX-homeodomain transcription factors. We describe the structure and expression of all eight P. vulgaris PvKNOX genes in both wild-type and Oakleaf plants, and present comparative transcriptome analysis of leaves and flowers from Oakleaf and wild-type plants. Oakleaf provides a new phenotypic marker for genetic analysis of the Primula S locus. We show that none of the Class I PvKNOX genes are strongly upregulated in Oakleaf leaves and flowers, and identify cohorts of 507 upregulated and 314 downregulated genes in the Oakleaf mutant
Beschreibung:Date Completed 01.06.2016
Date Revised 25.03.2024
published: Print-Electronic
GENBANK: KM586810, KM586811, KM586812, KM586813, KM586814, KM586815, KM586816, KM586817
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.13370