Comprehensive multiphase NMR a promising technology to study plants in their native state

Comprehensive multiphase NMR : a promising technology to study plants in their native state

Copyright © 2015 John Wiley & Sons, Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Magnetic resonance in chemistry : MRC. - 1985. - 53(2015), 9 vom: 07. Sept., Seite 735-44
1. Verfasser: Wheeler, Heather L (VerfasserIn)
Weitere Verfasser: Soong, Ronald, Courtier-Murias, Denis, Botana, Adolfo, Fortier-Mcgill, Blythe, Maas, Werner E, Fey, Michael, Hutchins, Howard, Krishnamurthy, Sridevi, Kumar, Rajeev, Monette, Martine, Stronks, Henry J, Campbell, Malcolm M, Simpson, Andre
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Magnetic resonance in chemistry : MRC
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 13C 1H Arabidopsis thaliana NMR cell wall ectopic lignification1 (eli1) lignin multiphase analysis mehr... natural samples plant Fatty Acids Nucleic Acids Water 059QF0KO0R Glutamine 0RH81L854J Phenylalanine 47E5O17Y3R Cellulose 9004-34-6 Starch 9005-25-8 Methanol Y4S76JWI15
Beschreibung
Zusammenfassung:Copyright © 2015 John Wiley & Sons, Ltd.
Nuclear magnetic resonance (NMR) spectroscopy is arguably one the most powerful tools to study the interactions and molecular structure within plants. Traditionally, however, NMR has developed as two separate fields, one dealing with liquids and the other dealing with solids. Plants in their native state contain components that are soluble, swollen, and true solids. Here, a new form of NMR spectroscopy, developed in 2012, termed comprehensive multiphase (CMP)-NMR is applied for plant analysis. The technology composes all aspects of solution, gel, and solid-state NMR into a single NMR probe such that all components in all phases in native unaltered samples can be studied and differentiated in situ. The technology is evaluated using wild-type Arabidopsis thaliana and the cellulose-deficient mutant ectopic lignification1 (eli1) as examples. Using CMP-NMR to study intact samples eliminated the bias introduced by extraction methods and enabled the acquisition of a more complete structural and metabolic profile; thus, CMP-NMR revealed molecular differences between wild type (WT) and eli1 that could be overlooked by conventional methods. Methanol, fatty acids and/or lipids, glutamine, phenylalanine, starch, and nucleic acids were more abundant in eli1 than in WT. Pentaglycine was present in A. thaliana seedlings and more abundant in eli1 than in WT
Beschreibung:Date Completed 16.05.2016
Date Revised 15.08.2015
published: Print-Electronic
Citation Status MEDLINE
ISSN:1097-458X
DOI:10.1002/mrc.4230