Nanopore Analysis of Single-Stranded Binding Protein Interactions with DNA

We study the binding of E. coli single-stranded binding protein (SSB) to single-stranded DNA (ssDNA) using a solid-state nanopore assay. We find that saturated nucleoprotein complexes can be distinguished easily from free SSB, ssDNA, or double-stranded DNA individually and demonstrate that the high...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1999. - 31(2015), 15 vom: 21. Apr., Seite 4582-8
1. Verfasser: Marshall, Michael M (VerfasserIn)
Weitere Verfasser: Ruzicka, Jan, Zahid, Osama K, Henrich, Vincent C, Taylor, Ethan W, Hall, Adam R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Single-Stranded DNA-Binding Proteins Escherichia coli Proteins Nucleoproteins