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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b00253
|2 doi
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|a pubmed24n0825.xml
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|a (DE-627)NLM247567124
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|a (NLM)25823528
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Decandio, Carla C
|e verfasserin
|4 aut
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| 245 |
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|a Self-Assembly of a Designed Alternating Arginine/Phenylalanine Oligopeptide
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.01.2016
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|a Date Revised 21.04.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A model octapeptide peptide consisting of an alternating sequence of arginine (Arg) and phenylalanine (Phe) residues, namely, [Arg-Phe]4, was prepared, and its self-assembly in solution studied. The simple alternating [Arg-Phe]4 peptide sequence allows for unique insights into the aggregation process and the structure of the self-assembled motifs. Fluorescence and UV-vis assays were used to determine critical aggregation concentrations, corresponding to the formation of oligomeric species and β-sheet rich structures organized into both spheroidal aggregates and highly ordered fibrils. Electron and atomic force microscopy images show globular aggregates and long unbranched fibers with diameters ranging from ∼4 nm up to ∼40 nm. Infrared and circular dichroism spectroscopy show the formation of β-sheet structures. X-ray diffraction on oriented stalks show that the peptide fibers have an internal lamellar structure, with an orthorhombic unit cell with parameters a ∼ 27.6 Å, b ∼ 9.7 Å, and c ∼ 9.6 Å. In situ small-angle X-ray scattering (SAXS) shows the presence of low molecular weight oligomers in equilibrium with mature fibers which are likely made up from 5 or 6 intertwined protofilaments. Finally, weak gel solutions are probed under gentle shear, suggesting the ability of these arginine-rich fibers to form networks
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gels
|2 NLM
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|a Oligopeptides
|2 NLM
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|a Protein Aggregates
|2 NLM
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|a Solutions
|2 NLM
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|a Phenylalanine
|2 NLM
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|a 47E5O17Y3R
|2 NLM
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| 650 |
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|a Arginine
|2 NLM
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| 650 |
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|a 94ZLA3W45F
|2 NLM
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| 700 |
1 |
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|a Silva, Emerson R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hamley, Ian W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Castelletto, Valeria
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liberato, Michelle S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Oliveira, Vani X
|c Jr
|e verfasserin
|4 aut
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|a Oliveira, Cristiano L P
|e verfasserin
|4 aut
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|a Alves, Wendel A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 31(2015), 15 vom: 21. Apr., Seite 4513-23
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:31
|g year:2015
|g number:15
|g day:21
|g month:04
|g pages:4513-23
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|u http://dx.doi.org/10.1021/acs.langmuir.5b00253
|3 Volltext
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|d 31
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|h 4513-23
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