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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2015.02.007
|2 doi
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|a pubmed24n0825.xml
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|a (DE-627)NLM24750789X
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|a (NLM)25817412
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|a (PII)S0176-1617(15)00047-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Navarro, Sara
|e verfasserin
|4 aut
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|a Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 26.01.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier GmbH. All rights reserved.
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|a Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44 kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44 kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), revealing a progressive increase of this metabolite throughout storage at 0 °C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cold storage
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|a Dehydrin gene expression
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|a Dehydrin isoforms
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|a Trehalose
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|a Vitis vinifera
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|a Phosphoproteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Protein Isoforms
|2 NLM
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|a RNA, Messenger
|2 NLM
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|a dehydrin proteins, plant
|2 NLM
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|a 134711-03-8
|2 NLM
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|a Carbon Dioxide
|2 NLM
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|a 142M471B3J
|2 NLM
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|a Trehalose
|2 NLM
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|a B8WCK70T7I
|2 NLM
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|a Vazquez-Hernandez, María
|e verfasserin
|4 aut
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|a Rosales, Raquel
|e verfasserin
|4 aut
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|a Sanchez-Ballesta, María Teresa
|e verfasserin
|4 aut
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|a Merodio, Carmen
|e verfasserin
|4 aut
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| 700 |
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|a Escribano, María Isabel
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 179(2015) vom: 01. Mai, Seite 1-11
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:179
|g year:2015
|g day:01
|g month:05
|g pages:1-11
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|u http://dx.doi.org/10.1016/j.jplph.2015.02.007
|3 Volltext
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