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231224s2015 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la505013u
|2 doi
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|a eng
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| 100 |
1 |
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|a Takada, Tadao
|e verfasserin
|4 aut
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| 245 |
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|a Photoresponsive DNA monolayer prepared by primer extension reaction on the electrode
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 04.01.2016
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|a Date Revised 07.04.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We describe a simple and convenient method for the preparation of photoresponsive DNA-modified electrodes using primer extension (PEX) reactions. A naphthalimide derivative was used as the photosensitizer that was attached to the C5-position of 2'-deoxyuridine-5'-triphosphate (dUTP(NI)). It has been found that dUTP(NI) is a good substrate for the PEX reactions using KOD Dash and Vent (exo-) enzymes in solutions to incorporate naphthalimide (NI) moieties into the DNA sequences. On the electrode surface immobilized with the primer/template DNA, the PEX reactions to incorporate dUTP(NI) molecules into the DNA sequence were found to efficiently proceed. With this solid-phase method, the DNA monolayers capable of generating photocurrent due to the photoresponsive NI molecule can be constructed. It was shown that the photocurrent generation was significantly suppressed by a single-nucleotide mismatch included in the primer/template DNA, which is applicable for the design of photoelectrochemical sensors to discriminate single-nucleotide sequences
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Deoxyuracil Nucleotides
|2 NLM
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|a deoxyuridine triphosphate
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|a 1173-82-6
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|a DNA
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|a 9007-49-2
|2 NLM
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|a Takemura, Mai
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|a Kawano, Yuta
|e verfasserin
|4 aut
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|a Nakamura, Mitsunobu
|e verfasserin
|4 aut
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|a Yamana, Kazushige
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 31(2015), 13 vom: 07. Apr., Seite 3993-8
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|x 1520-5827
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| 773 |
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|g volume:31
|g year:2015
|g number:13
|g day:07
|g month:04
|g pages:3993-8
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|u http://dx.doi.org/10.1021/la505013u
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