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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2015.03.010
|2 doi
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|a pubmed24n0824.xml
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|a (DE-627)NLM247393525
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|a (NLM)25805654
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|a (PII)S1521-6616(15)00110-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Tajima, Asako
|e verfasserin
|4 aut
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| 245 |
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|a Bioengineering mini functional thymic units with EAK16-II/EAKIIH6 self-assembling hydrogel
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.12.2015
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|a Date Revised 10.06.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Inc. All rights reserved.
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|a Herein, we highlight the technical feasibility of generating a functional mini thymus with a novel hydrogel system, based on a peptide-based self-assembly platform that can induce the formation of 3-D thymic epithelial cell (TEC) clusters. Amphiphilic peptide EAK16-II co-assembled with its histidinylated analogue EAKIIH6 into beta-sheet fibrils. When adaptor complexes (recombinant protein A/G molecules loaded with both anti-His and anti-EpCAM IgGs) were added to the mix, TECs were tethered to the hydrogel and formed 3-D mini clusters. TECs bound to the hydrogel composites retained their molecular properties; and when transplanted into athymic nude mice, they supported the development of functional T-cells. These mini thymic units of TECs can be useful in clinical applications to reconstitute T-cell adaptive immunity
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a EAK16-II
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|a Hydrogel
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|a Self-assembly
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|a Thymic epithelial cells
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|a Thymopoiesis
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| 650 |
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|a Thymus
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|a EAK16 peptide
|2 NLM
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|a Hydrogels
|2 NLM
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|a Oligopeptides
|2 NLM
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| 650 |
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|a Peptides
|2 NLM
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| 700 |
1 |
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|a Liu, Wen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pradhan, Isha
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bertera, Suzanne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bagia, Christina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Trucco, Massimo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meng, Wilson S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fan, Yong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 160(2015), 1 vom: 15. Sept., Seite 82-9
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
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|g volume:160
|g year:2015
|g number:1
|g day:15
|g month:09
|g pages:82-9
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|u http://dx.doi.org/10.1016/j.clim.2015.03.010
|3 Volltext
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