Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 66(2015), 11 vom: 01. Juni, Seite 3041-54
1. Verfasser:	Qiu, Zongbo (VerfasserIn)
Weitere Verfasser:	Li, Xiaojuan, Zhao, Yuanyuan, Zhang, Manman, Wan, Yinglang, Cao, Dechang, Lu, Shanfa, Lin, Jinxing
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Chinese fir (Cunninghamia lanceolata) high-throughput small RNA sequencing microRNA vascular cambium wood formation. MicroRNAs RNA, Plant RNA, Small Untranslated

Beschreibung
Zusammenfassung:	© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. MicroRNAs (miRNAs) are small noncoding regulatory RNAs that play key roles in the process of plant development. To date, extensive studies of miRNAs have been performed in a few model plants, but few efforts have focused on small RNAs (sRNAs) in conifers because of the lack of reference sequences for their enormous genomes. In this study, Solexa sequencing of three sRNA libraries obtained from dormant, reactivating, and active vascular cambium in Chinese ﬁr (Cunninghamia lanceolata) using tangential cryosectioning identified 20 known miRNA families and 18 novel potential miRNAs, of which nine novel miRNA precursors were validated by RT-PCR and sequencing. More than half of these novel miRNAs displayed stage-specific expression patterns in the vascular cambium. Furthermore, analysing the 103 miRNAs and their predicted targets indicated that about 70% appeared to negatively regulate their targets, of which two target genes involved in the regulation of cambial cell division were validated via RNA ligase-mediated rapid amplification of 5'-cDNA ends (RLM 5'-RACE) and transient co-expression in Nicotiana benthamiana leaves. Interestingly, miRNA156 and miRNA172 may regulate the phase transition in vascular cambium from dormancy to active growth. These results provide new insights into the important regulatory functions of miRNAs in vascular cambium development and wood formation in conifers
Beschreibung:	Date Completed 30.03.2016 Date Revised 13.12.2023 published: Print-Electronic GEO: GSE53933 Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erv103