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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2015.03.001
|2 doi
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|a pubmed24n0823.xml
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|a (DE-627)NLM247065137
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|a (NLM)25769244
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|a (PII)S1521-6616(15)00091-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Pahar, Bapi
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Transforming growth factor-β1 regulated phosphorylated AKT and interferon gamma expressions are associated with epithelial cell survival in rhesus macaque colon explants
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.07.2015
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|a Date Revised 13.11.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Inc. All rights reserved.
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|a Transforming growth factor-β1 (TGF-β1) is an important immunoregulatory cytokine that plays an obligate role in regulating T-cell functions. Here, we demonstrated the role of TGF-β1 in regulating the survival of intestinal epithelial cells (ECs) in rhesus colon explant cultures using either anti-TGF-β1 antibody or recombinant TGF-β1 proteins. Neutralization of endogenous TGF-β1 using anti-TGF-β1 antibodies induced apoptosis of both intestinal ECs and lamina propria (LP) cells. Additionally, endogenous TGF-β1 blocking significantly increased expression of IFNγ, TNFα, CD107a and Perforin in LP cells compared to media and isotype controls. A significant decrease in pAKT expression was detected in anti-TGF-β1 MAbs treated explants compared to isotype and rTGF-β1 protein treated explants. Our results demonstrated TGF-β1 regulated pAKT and IFNγ expressions were associated with epithelial cell survival in rhesus macaque colon explants and suggest a potential role of mucosal TGF-β1 in regulating intestinal homeostasis and EC integrity
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Apoptosis
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|a Colon
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|a Epithelial cell
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|a Rhesus macaque
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|a TGF-β1
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|a pAKT
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| 650 |
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|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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|a Transforming Growth Factor beta1
|2 NLM
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| 650 |
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|a Interferon-gamma
|2 NLM
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| 650 |
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|a 82115-62-6
|2 NLM
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| 650 |
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|a Proto-Oncogene Proteins c-akt
|2 NLM
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| 650 |
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|a EC 2.7.11.1
|2 NLM
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| 700 |
1 |
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|a Pan, Diganta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lala, Wendy
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kenway-Lynch, Carys S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Das, Arpita
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 158(2015), 1 vom: 26. Mai, Seite 8-18
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:158
|g year:2015
|g number:1
|g day:26
|g month:05
|g pages:8-18
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|u http://dx.doi.org/10.1016/j.clim.2015.03.001
|3 Volltext
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